Diacylglycerol kinase beta, Dg

Mouse Diacylglycerol kinase beta, Dgkb ELISA KIT

Product Name:Mouse Diacylglycerol kinase beta, Dgkb ELISA KIT
Packing:96T

Catalog No.:ELI-32053m

Gene Name:Mouse Dgkb

Detect Range:31.25-2000pg/ml

Sensitivity:18.75pg/ml

Target Protein Name:Mouse Dgkb

Alternative Name:Mouse Diacylglycerol kinase beta, Dgkb
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Diacylglycerol kinase beta, Dgkb ELISA KIT allows for the in vitro quantitative determination of Mouse Dgkb concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Diacylglycerol kinase beta, Dgkb ELISA KIT has been pre-coated with an Mouse Diacylglycerol kinase beta, Dgkb antibody specific to Mouse Dgkb .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dgkb and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dgkb , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dgkb in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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