ATP-dependent RNA helicase DDX
25, Ddx25 ELISA KIT/ Mouse ATP-dependent RNA helicase DDX25, Ddx25 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-32033m
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
ATP-dependent RNA helicase DDX25, Ddx25
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT
Product Name:Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT
Packing:96T
Catalog No.:ELI-32033m
Gene Name:Mouse Ddx25
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Ddx25
Alternative Name:Mouse ATP-dependent RNA helicase DDX25, Ddx25
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT allows for the in vitro quantitative determination of Mouse Ddx25 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse ATP- dependent RNA helicase DDX25, Ddx25 ELISA KIT has been pre-coated with an Mouse ATP-dependent RNA helicase DDX25, Ddx25 antibody specific to Mouse Ddx25 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ddx25 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ddx25 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ddx25 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验following manufacturers protocol.In vitro transcribed RNA probes(using Bartel lab T7 polymerase):1-2ulDNA template (~100-500ng)5ul10 X T7 buffer1ul10mM ATP1ul10mM CTP1ul10mM GTP5ulalpha P32 UTP (800Ci/mmol) NEN cat# : BLU007X1ul0.25M DTT2.5ulT7 polymerase
机制已日渐清晰。Zamore 等利用果蝇胚胎提取物建立的体外系统, 证实RNAi 是一个依赖ATP 的过程, 在此过程中, dsRNA (外源的或体内产生的) 首先被降解为具5"2单磷酸、长21～23bp 的小分子双链RNA , 这种RNA 分子称为小干扰RNA , siRNA 通过碱基互补配对识别具同源序列的mRNA , 并介导其降解。研究表明在生物体中siRNA 具相似的结构特征: 为长约21～23bp 的双链RNA , 具5"单磷酸和3"羟基末端, 互补双链的3"端均有一个2～3nt 的单链突出
RNA Analysis by Nuclease Protection
Figure 5.1.2 Typical RNase protection assay using a probe for mouse β‐actin. A 300‐base mouse β‐actin probe was hybridized to two‐fold‐increasing amounts of mouse total‐liver RNA (from 0.625 to 10 µg; lanes 4 to 8) and analyzed by RNase protection
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