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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
DNA cross-link repair 1A protein, Dclre1a
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse DNA cross- link repair 1A protein, Dclre1a ELISA KIT
Product Name:Mouse DNA cross- link repair 1A protein, Dclre1a ELISA KIT
Packing:96T
Catalog No.:ELI-32026m
Gene Name:Mouse Dclre1a
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Dclre1a
Alternative Name:Mouse DNA cross-link repair 1A protein, Dclre1a
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse DNA cross- link repair 1A protein, Dclre1a ELISA KIT allows for the in vitro quantitative determination of Mouse Dclre1a concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse DNA cross- link repair 1A protein, Dclre1a ELISA KIT has been pre-coated with an Mouse DNA cross-link repair 1A protein, Dclre1a antibody specific to Mouse Dclre1a .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dclre1a and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dclre1a , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dclre1a in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DNA Interstrand Cross‐Link Formation Using Furan as a Masked Reactive Aldehyde
., Katinger H., and Voglauer, R. 2007. Contributions of DNA interstrand cross‐links to aging of cells and organisms. Nucleic Acids Res. 35:7566‐7576. Guainazzi, A. and Scharer, O.D
Syntheses of DNA Duplexes That Contain a N4C‐Alkyl‐N4C Interstrand Cross‐Link
., Paranawithana, S.R., Miller, P.S., and Kielkopf, C.L. 2007. Structure of a DNA repair substrate containing an alkyl interstrand cross‐link at 1.65 A resolution. Biochemistry 46:4545‐4553.
Methods to Study Transcription-Coupled Repair in Chromatin
-link proteins to DNA and enrichment of a protein of interest is done by subsequent immunoprecipitation. Upon reversal of the protein–DNA cross-links, the amount of coprecipitated DNA fragments can be detected by quantitative PCR. To perform ChIP on UV
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