- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-05162m
- 2025年12月30日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Integrin alpha-L, Itgal含量
- 适应物种：
小鼠
- 标记物：
Integrin alpha-L, Itgal
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Integrin alpha-L, Itgal ELISA试剂盒
Product Name: Mouse Integrin alpha-L, Itgal ELISA试剂盒
Product Name: Mouse Integrin alpha-L, Itgal ELISA试剂盒
Gene Name: Mouse Integrin alpha-L, Itgal
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Integrin alpha-L, Itgal
Alternative Name: Mouse Integrin alpha-L, Itgal
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Integrin alpha-L, Itgal ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Integrin alpha-L, Itgal concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Integrin alpha-L, Itgal ELISA试剂盒 has been pre-coated with an Mouse Integrin alpha-L, Itgal antibody specific to Mouse Integrin alpha-L, Itgal .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Integrin alpha-L, Itgal and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Integrin alpha-L, Itgal , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Integrin alpha-L, Itgal in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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