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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Large neutral amino acids transporter small subunit 1, Slc
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
SLC7A5,CD98 light chain; CD98; 4F2LC; D16S469E; E16; LAT1; MPE16; hLAT1; 4F2 LC; 4F2 light chain; L-type amino acid transporter 1; integral membrane protein E16; large neutral amino acids transporter 1; large neutral amino acids transporter small subunit 1; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; solute carrier family 7 member 5; y+ system cationic amino acid transporter; solute carrier family 7 (amino acid transporter light chain, L system), member 5
Search name
Mouse SLC7A5 ELISA KIT ,Mouse CD98 light chain ELISA KIT ,Mouse CD98 ELISA KIT ,Mouse 4F2LC ELISA KIT ,Mouse D16S469E ELISA KIT ,Mouse E16 ELISA KIT ,Mouse LAT1 ELISA KIT ,Mouse MPE16 ELISA KIT ,Mouse hLAT1 ELISA KIT ,Mouse 4F2 LC ELISA KIT ,Mouse 4F2 light chain ELISA KIT ,Mouse L-type amino acid transporter 1 ELISA KIT ,Mouse integral membrane protein E16 ELISA KIT ,Mouse large neutral amino acids transporter 1 ELISA KIT ,Mouse large neutral amino acids transporter small subunit 1 ELISA KIT ,Mouse sodium-independent neutral amino acid transporter LAT1 ELISA KIT ,Mouse solute carrier family 7 member 5 ELISA KIT ,Mouse solute carrier family 7 member 5 ELISA KIT ,Mouse y+ system cationic amino acid transporter ELISA KIT ,Mouse solute carrier family 7 member 5 ELISA KIT ,Mouse amino acid transporter light chain L system member 5 ELISA KIT ,Mouse cationic amino acid transporter y+ system member 5 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 beta concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse Large neutral amino acids transporter small subunit 1, SLC7A5. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Large neutral amino acids transporter small subunit 1, SLC7A5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Mouse Large neutral amino acids transporter small subunit 1, SLC7A5 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验are more than 30 amino acids away from all possible cleavage sites. The finding that CRP analysis is not applicable may in itself confine a phosphorylation site to a segment of the protein that is likely to produce very large proteolytic fragments. The Cleavage
of the same concentration, agarose is used to separate larger macromolecules such as nucleic acids, large proteins and protein complexes. Polyacrylamide, which is easy to handle and to make at higher concentrations, is used to separate most proteins and small
than the anode. Because of the amino acids in proteins, they have amphoteric propertites and will be positively charged at pH values below their IpH and negatively charged above. This means that proteins will migrate toward their IpH. Most proteins have a IpH
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