Ig gamma-1 chain C region secr

Mouse Ig gamma- 1 chain C region secreted form, Ighg1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

IGHG1,Ig gamma-1 chain C region,Igh-4,Ig gamma-1 chain C region secreted form, Ig gamma-1 chain C region, membrane-bound form

Search name

Mouse IGHG1 ELISA KIT ,Mouse Ig gamma-1 chain C region ELISA KIT ,Mouse Igh-4 ELISA KIT ,Mouse Ig gamma-1 chain C region secreted form ELISA KIT ,Mouse Ig gamma-1 chain C region membrane-bound form ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Ig gamma- 1 chain C region, IGHG1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to IGHG1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IGHG1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain IGHG1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IGHG1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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