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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Proteasome subunit beta type-11, Psmb11
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Proteasome subunit beta type- 11, Psmb11 ELISA KIT
Product Name:Mouse Proteasome subunit beta type- 11, Psmb11 ELISA KIT
Packing:96T
Catalog No.:ELI-30598m
Gene Name:Mouse Psmb11
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Psmb11
Alternative Name:Mouse Proteasome subunit beta type-11, Psmb11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Proteasome subunit beta type- 11, Psmb11 ELISA KIT allows for the in vitro quantitative determination of Mouse Psmb11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Proteasome subunit beta type- 11, Psmb11 ELISA KIT has been pre-coated with an Mouse Proteasome subunit beta type-11, Psmb11 antibody specific to Mouse Psmb11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Psmb11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Psmb11 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Psmb11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
or other proteasome inhibitor (e.g., lactacystin; Calbiochem or Biomol) Phosphate‐buffered saline (PBS; see recipe ) Nondenaturing Triton X‐100 lysis buffer (see recipe ) containing 1×
In Vitro Reconstitution of CFTR Biogenesis and Degradation
is recognized by a large multicatalytic protease, the 26S proteasome. This proteolytic machine is formed through the ATP-dependent association of two multimeric components, the 20S proteasome and the 19S regulatory subunit. The 19S subunit recognizes
Genetic and Pharmacologic Models for Type 1 Diabetes
without autoimmune involvement, as do a variety of transgenes overexpressed in beta cells. Pharmacologically induced T1D (without autoimmunity) elicited by alloxan or streptozotocin at high doses can generate hyperglycemia in almost any strain of mouse by direct
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