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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cytochrome b-245 light chain, Cyba
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cytochrome b- 245 light chain, Cyba ELISA KIT
Product Name:Mouse Cytochrome b- 245 light chain, Cyba ELISA KIT
Packing:96T
Catalog No.:ELI-32853m
Gene Name:Mouse Cyba
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cyba
Alternative Name:Mouse Cytochrome b-245 light chain, Cyba
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cytochrome b- 245 light chain, Cyba ELISA KIT allows for the in vitro quantitative determination of Mouse Cyba concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cytochrome b- 245 light chain, Cyba ELISA KIT has been pre-coated with an Mouse Cytochrome b-245 light chain, Cyba antibody specific to Mouse Cyba .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cyba and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cyba , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cyba in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验实验步骤 1. Using the AbC™ Anti-Mouse Bead Kit 1) Completely resuspend the AbC™ capture beads (Component A) and negative beads (Component B) by gently vortexing for 30 seconds before use. 2) Label
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
as follows: Distilled water 1 ml Reagent B1 1 drop Reagent B2 1 drop Reagent B3 1 drop Mix well. Protect from light and use
8 for that tube. 10) Resuspend the bead pellet by adding 0.5 mL of buffer to each sample tube. 11) Add one drop of negative beads (Component B in the AbC™ Anti-Mouse Bead Kit) to sample tube(s) containing the AbC™ capture beads. 12) Add
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