Ubiquitin carboxyl-terminal hy
drolase 49, Usp49 ELISA KIT/ Mouse Ubiquitin carboxyl-terminal hydrolase 49, Usp49 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-28828m
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ubiquitin carboxyl-terminal hydrolase 49, Usp49
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ubiquitin carboxyl- terminal hydrolase 49, Usp49 ELISA KIT
Product Name:Mouse Ubiquitin carboxyl- terminal hydrolase 49, Usp49 ELISA KIT
Packing:96T
Catalog No.:ELI-28828m
Gene Name:Mouse Usp49
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Usp49
Alternative Name:Mouse Ubiquitin carboxyl-terminal hydrolase 49, Usp49
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin carboxyl- terminal hydrolase 49, Usp49 ELISA KIT allows for the in vitro quantitative determination of Mouse Usp49 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin carboxyl- terminal hydrolase 49, Usp49 ELISA KIT has been pre-coated with an Mouse Ubiquitin carboxyl-terminal hydrolase 49, Usp49 antibody specific to Mouse Usp49 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Usp49 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Usp49 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Usp49 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Membrane‐Based Yeast Two‐Hybrid System to Detect Protein Interactions
to NubG, the mutated N‐terminal half of ubiquitin. NubG may be fused either to the N‐terminus of the prey (NubG‐ x ) or to the C‐terminus ( x ‐NubG). (D ) If bait and prey interact, Cub and NubG are forced into close proximity and reassemble to form split
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