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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Filaggrin-2, Flg2
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Filaggrin- 2, Flg2 ELISA KIT
Product Name:Mouse Filaggrin- 2, Flg2 ELISA KIT
Packing:96T
Catalog No.:ELI-27508m
Gene Name:Mouse Flg2
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Flg2
Alternative Name:Mouse Filaggrin-2, Flg2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Filaggrin- 2, Flg2 ELISA KIT allows for the in vitro quantitative determination of Mouse Flg2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Filaggrin- 2, Flg2 ELISA KIT has been pre-coated with an Mouse Filaggrin-2, Flg2 antibody specific to Mouse Flg2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Flg2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Flg2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Flg2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
意义。AKA的出现不仅有助于RA的诊断,还可通过动态检测血清AKA滴度的变化来估计其疾病活动性和预后。 2、抗核周因子抗体(APF) 1964年荷兰学者Nieenhuis和Mandema首次以人颊粘膜上皮细胞为抗原底物,用间接免疫荧光法(IIF)检测RA血清中的抗核周因子抗体。APF是一种RA特异性的免疫球蛋白,以IgG为主,现在学者多数认为APF所针对的抗原存在于人颊粘膜细胞的角质透明蛋白颗粒,其成分可能是上皮细胞的中等纤维结合蛋白或其前体,即(pro)Filaggrin,APF对RA
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