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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cullin-1, Cul1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cullin- 1, Cul1 ELISA KIT
Product Name:Mouse Cullin- 1, Cul1 ELISA KIT
Packing:96T
Catalog No.:ELI-26494m
Gene Name:Mouse Cul1
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Cul1
Alternative Name:Mouse Cullin-1, Cul1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cullin- 1, Cul1 ELISA KIT allows for the in vitro quantitative determination of Mouse Cul1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cullin- 1, Cul1 ELISA KIT has been pre-coated with an Mouse Cullin-1, Cul1 antibody specific to Mouse Cul1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cul1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cul1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cul1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
利用效率和抗旱能力。 生长素是一类低分子量的植物激素,它通过调节细胞分裂、伸长和分化对植物生长发育的各个方面发挥重要的调节作用。在拟南芥中生长素通过依赖于泛素分子的蛋白降解途径发挥功能,调控下游基因表达。早在1993年人们鉴定到拟南芥分枝增加的突变体axr1, 发现AXR1蛋白参与依赖于泛素分子的蛋白降解途径[2]。AXR1能够激活泛素样蛋白RUB1,并促进RUB1与SCFTIR1复合体中CUL1蛋白的结合。AXR1蛋白的突变使RUB1与CUL1的结合降低,SCF复合体功能降低,进而带来
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