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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Cyclin-dependent kinase 10, Cdk10
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Cyclin- dependent kinase 10, Cdk10 ELISA KIT
Product Name:Mouse Cyclin- dependent kinase 10, Cdk10 ELISA KIT
Packing:96T
Catalog No.:ELI-25430m
Gene Name:Mouse Cdk10
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Cdk10
Alternative Name:Mouse Cyclin-dependent kinase 10, Cdk10
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Cyclin- dependent kinase 10, Cdk10 ELISA KIT allows for the in vitro quantitative determination of Mouse Cdk10 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Cyclin- dependent kinase 10, Cdk10 ELISA KIT has been pre-coated with an Mouse Cyclin-dependent kinase 10, Cdk10 antibody specific to Mouse Cdk10 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cdk10 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cdk10 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cdk10 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
Cell-Free Assay System for Ras- and Rap1-Dependent Activation of MAP-Kinase Cascade
It is well-established that Ras activates the mitogen-activated protein (MAP) kinase cascade consisting of MAP kinase, extracellular signal-regulated kinase (ERK), ERK kinase (MEK), and MEK kinase in mammals (for reviews, see refs. 1 –
Analysis of Opioid-Induced Kinase Activation
(ATP) onto the serine, threonine, or tyrosine residue. It has been shown that stimulation of opioid receptors regulates activities of numerous protein kinases including protein kinase C (PKC), cAMP-dependent protein kinase (PKA), Ca2+ /calmodulin
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