- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-25242m
- 2025年07月05日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Carbonic anhydrase 13, Ca13
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Carbonic anhydrase 13, Ca13 ELISA KIT
Product Name:Mouse Carbonic anhydrase 13, Ca13 ELISA KIT
Packing:96T
Catalog No.:ELI-25242m
Gene Name:Mouse Ca13
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Ca13
Alternative Name:Mouse Carbonic anhydrase 13, Ca13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Carbonic anhydrase 13, Ca13 ELISA KIT allows for the in vitro quantitative determination of Mouse Ca13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Carbonic anhydrase 13, Ca13 ELISA KIT has been pre-coated with an Mouse Carbonic anhydrase 13, Ca13 antibody specific to Mouse Ca13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ca13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ca13 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ca13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【Jounal club】NATURE MEDICINE: 利用蛋白组技术发现糖尿病眼底病变新机制
News and Views: A prize catch for diabetic retinopathy Nature Medicine - 13, 131 - 132 (2007) Article: Extracellular carbonic anhydrase mediates hemorrhagic retinal and cerebral vascular permeability through prekallikrein activation Nature
dehydrogenase ， LDH ）、碳酸酐酶（ carbonic anhydrase ）及其它氧化还原酶类（ oxidoreductases ）等均属结合蛋白酶。这些酶除了蛋白质组分外，还含对热稳定的非蛋白小分子物质。前者称为酶蛋白（ apoenzyme ），后者称为辅因子（ cofacters ）。酶蛋白与辅因子单独存在时，均无催化活力。只有二者结合成完整的分子时，才具有酶活力。此完整的酶分子称为全酶（ holoenzyme ）。 全酶 = 酶蛋白 ＋ 辅因子
羧肽酶 A[carboxypeptidase A] 碳酸酐酶[carbonic anhydrase] 枯草杆菌蛋白酶[subtilisin] γ-球蛋白，L链γ-blobulin,L chain[] 糜蛋白酶原（胰凝乳蛋白酶原）[chymotrypsinogen 胰蛋白酶[trypsin] 木瓜蛋白酶（羧甲基）[papain (carboxymethyl)] β-乳球蛋白[&beta
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