SH3 domain-binding protein 5-l
ike, Sh3bp5l ELISA KIT/ Mouse SH3 domain-binding protein 5-like, Sh3bp5l ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-24703m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
SH3 domain-binding protein 5-like, Sh3bp5l
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse SH3 domain- binding protein 5- like, Sh3bp5l ELISA KIT
Product Name:Mouse SH3 domain- binding protein 5- like, Sh3bp5l ELISA KIT
Packing:96T
Catalog No.:ELI-24703m
Gene Name:Mouse Sh3bp5l
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Sh3bp5l
Alternative Name:Mouse SH3 domain-binding protein 5-like, Sh3bp5l
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse SH3 domain- binding protein 5- like, Sh3bp5l ELISA KIT allows for the in vitro quantitative determination of Mouse Sh3bp5l concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse SH3 domain- binding protein 5- like, Sh3bp5l ELISA KIT has been pre-coated with an Mouse SH3 domain-binding protein 5-like, Sh3bp5l antibody specific to Mouse Sh3bp5l .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Sh3bp5l and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Sh3bp5l , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Sh3bp5l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mapping the Specificity of SH3 Domains with Phage-Displayed Random-Peptide Libraries
for a variety of modular domains (3 –8 ). Ligands derived from these libraries often resemble sequences in natural-binding proteins. Thus, combinatorral-peptide libraries provide a means of mapping modular domain-mediated protein-protein interactions
Phosphotyrosine Kinase Assays as a Prescreen for Inhibitors of EGFr
of the same protein (4 ). In the absence of EGF, tyrosine kinase activity is low. Binding of EGF to the receptor domain results in receptor dimerisation and subsequent autophosphorylation of the cytoplasmic domain of EGFr at specific tyrosine residues
Use of Peptide Libraries to Determine Optimal Substrates of Tyrosine Kinases
(3 ). Nonreceptor tyrosine kinases such as Src and Abl are also targeted to subsets of cellular proteins by virtue of specific interactions with their SH2 and SH3 domains. The Src SH3 domain recognizes a proline-rich motif in an actin filament-assoctated protein
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