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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Breakpoint cluster region protein, Bcr
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Breakpoint cluster region protein, Bcr ELISA KIT
Product Name:Mouse Breakpoint cluster region protein, Bcr ELISA KIT
Packing:96T
Catalog No.:ELI-24483m
Gene Name:Mouse Bcr
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Bcr
Alternative Name:Mouse Breakpoint cluster region protein, Bcr
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Breakpoint cluster region protein, Bcr ELISA KIT allows for the in vitro quantitative determination of Mouse Bcr concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Breakpoint cluster region protein, Bcr ELISA KIT has been pre-coated with an Mouse Breakpoint cluster region protein, Bcr antibody specific to Mouse Bcr .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Bcr and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Bcr , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Bcr in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验A Nested RT-PCR Assay to Detect BCR/abl
) (1 ). In this chromosomal translocation, exons from a major breakpoint cluster region (M-bcr), located on chromosome 22q11, are joined to the c-abl protooncogene, located on chromosome 9q34. When this chromosomal translocation occurs in a hematopoietic stem cell
A Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/abl
) (1) . In this chromosomal translocation, exons from a major breakpoint cluster region (M-bcr), located on chromosome 22q11, are joined to the c-abl proto-oncogene, located on chromosome 9q34. When this chromosomal translocation occurs in a hematopoietic stem cell
Real-Time Quantitative Reverse Transcriptase Polymerase Chain Reaction
and is characterized by a balanced (9;22) chromosomal translocation that results in the formation of a chimeric gene comprised of the BCR (breakpoint cluster region) gene and the ABL oncogene (BCR-ABL fusion gene). The chimeric gene encodes a fusion protein
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