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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Autophagy-related protein 2 homolog B, Atg2b
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Autophagy- related protein 2 homolog B, Atg2b ELISA KIT
Product Name:Mouse Autophagy- related protein 2 homolog B, Atg2b ELISA KIT
Packing:96T
Catalog No.:ELI-24102m
Gene Name:Mouse Atg2b
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Atg2b
Alternative Name:Mouse Autophagy-related protein 2 homolog B, Atg2b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Autophagy- related protein 2 homolog B, Atg2b ELISA KIT allows for the in vitro quantitative determination of Mouse Atg2b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Autophagy- related protein 2 homolog B, Atg2b ELISA KIT has been pre-coated with an Mouse Autophagy-related protein 2 homolog B, Atg2b antibody specific to Mouse Atg2b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Atg2b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Atg2b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Atg2b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
and stable gene expression in human embryonic stem (ES) cells. Here, we describe a method for transfecting and establishing stable cell lines in mouse embryonic stem (mES) cells with the SB system. RELATED INFORMATION See Figure
3) Get the protein sequence of this gene, copy and paste and save the sequence (do a good annotation in your .doc or .txt file, so that you can always go back and find out what exactly you are looking at. Part B: Do BLAST search to find Drosophila homolog
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