Peripheral myelin protein 22,
Pmp22 ELISA KIT/ Mouse Peripheral myelin protein 22, Pmp22 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22966m
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Peripheral myelin protein 22, Pmp22
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Peripheral myelin protein 22, Pmp22 ELISA KIT
Product Name:Mouse Peripheral myelin protein 22, Pmp22 ELISA KIT
Packing:96T
Catalog No.:ELI-22966m
Gene Name:Mouse Pmp22
Detect Range:31.25-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pmp22
Alternative Name:Mouse Peripheral myelin protein 22, Pmp22
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Peripheral myelin protein 22, Pmp22 ELISA KIT allows for the in vitro quantitative determination of Mouse Pmp22 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Peripheral myelin protein 22, Pmp22 ELISA KIT has been pre-coated with an Mouse Peripheral myelin protein 22, Pmp22 antibody specific to Mouse Pmp22 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pmp22 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pmp22 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pmp22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验The COS-7 Cell In Vitro Paradigm to Study Myelin Proteolipid Protein 1 Gene Mutations
of proteolipid protein 1 (PLP1) in transiently transfected COS-7 cells. In humans, the PLP1 gene is located on the long arm of the X-chromosome and deletion, duplication, or coding region mutations in this gene cause the leukodystrophy, Pelizaeus-Merzbacher
Strategies to Optimize Protein Expression in E. coli
. 2007. Purification and initiation of structural characterization of human peripheral myelin protein 22, an integral membrane protein linked to peripheral neuropathies. Biochemistry 46:11185‐11195.
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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