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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Keratin type I cytoskeletal 20, KRT20含量
- 适应物种:
小鼠
- 标记物:
Keratin type I cytoskeletal 20, KRT20
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Keratin type I cytoskeletal 20, KRT20 ELISA试剂盒
Product Name: Mouse Keratin type I cytoskeletal 20, KRT20 ELISA试剂盒
Product Name: Mouse Keratin type I cytoskeletal 20, KRT20 ELISA试剂盒
Gene Name: Mouse Keratin type I cytoskeletal 20, KRT20
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Keratin type I cytoskeletal 20, KRT20
Alternative Name: Mouse Keratin type I cytoskeletal 20, KRT20
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Keratin type I cytoskeletal 20, KRT20 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Keratin type I cytoskeletal 20, KRT20 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Keratin type I cytoskeletal 20, KRT20 ELISA试剂盒 has been pre-coated with an Mouse Keratin type I cytoskeletal 20, KRT20 antibody specific to Mouse Keratin type I cytoskeletal 20, KRT20 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Keratin type I cytoskeletal 20, KRT20 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Keratin type I cytoskeletal 20, KRT20 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Keratin type I cytoskeletal 20, KRT20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
of interest as the solvents can partially dissolve indigo. For glutaraldehyde-fixed mouse retina, which is approximately 250 microns thick, the following procedure was used. Dehydrate through graded ethanols (50%, 70%, 95%, 100%, 100%) for 20 min each. Clear in xylene, 2 x 15
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