Ubiquitin-conjugating enzyme E
2 S, Ube2s ELISA KIT/ Mouse Ubiquitin-conjugating enzyme E2 S, Ube2s ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-22299m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ubiquitin-conjugating enzyme E2 S, Ube2s
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT
Product Name:Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT
Packing:96T
Catalog No.:ELI-22299m
Gene Name:Mouse Ube2s
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ube2s
Alternative Name:Mouse Ubiquitin-conjugating enzyme E2 S, Ube2s
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT allows for the in vitro quantitative determination of Mouse Ube2s concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ubiquitin- conjugating enzyme E2 S, Ube2s ELISA KIT has been pre-coated with an Mouse Ubiquitin-conjugating enzyme E2 S, Ube2s antibody specific to Mouse Ube2s .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ube2s and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ube2s , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ube2s in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
Stability and Ubiquitination of the Tumor Suppressor Protein p53
-dependent manner through its covalent thio-ester linkage to the E1 ubiquitin-activating enzyme. The activated ubiquitin is then transferred to the E2 enzyme, also known as a ubiquitin conjugating enzyme (UBC), again in the form of a high-energy thio-ester
Examining Protein Stability and Its Relevance for Plant Growth and Development
the poly-ubiquitylation of degradation targets by E1 ubiquitin activating enzymes, E2 ubiquitin conjugating enzymes, and E3 ubiquitin ligases. Specificity is brought to the process by E3 ubiquitin ligases, which engage in direct interactions
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