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- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
UTP--glucose-1-phosphate uridylyltransferase, Ugp2 ELI
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse UTP- - glucose- 1- phosphate uridylyltransferase, UGP2 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
UGP2,UDPG; UDPGP; UDPGP2; UGP1; UGPP1; UGPP2; pHC379; UDP-glucose diphosphorylase; UDP-glucose pyrophosphorylase 1; UGPase 2; UTP--glucose-1-phosphate uridylyltransferase; UTP--glucose-1-phosphate uridylyltransferase 2; UTP-glucose-1-phosphate uridyltransferase; Uridyl diphosphate glucose pyrophosphorylase-1; uridyl diphosphate glucose pyrophosphorylase 2; UDP-glucose pyrophosphorylase 2
Search name
Mouse UGP2 ELIAS KIT,Mouse UDPG ELIAS KIT,Mouse UDPGP ELIAS KIT,Mouse UDPGP2 ELIAS KIT,Mouse UGP1 ELIAS KIT,Mouse UGPP1 ELIAS KIT,Mouse UGPP2 ELIAS KIT,Mouse pHC379 ELIAS KIT,Mouse UDP-glucose diphosphorylase ELIAS KIT,Mouse UDP-glucose pyrophosphorylase 1 ELIAS KIT,Mouse UGPase 2 ELIAS KIT,Mouse UTP--glucose-1-phosphate uridylyltransferase ELIAS KIT,Mouse UTP--glucose-1-phosphate uridylyltransferase 2 ELIAS KIT,Mouse UTP-glucose-1-phosphate uridyltransferase ELIAS KIT,Mouse Uridyl diphosphate glucose pyrophosphorylase-1 ELIAS KIT,Mouse uridyl diphosphate glucose pyrophosphorylase 2 ELIAS KIT,Mouse UDP-glucose pyrophosphorylase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse UTP- - glucose- 1- phosphate uridylyltransferase, UGP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to UDP-glucose pyrophosphorylase, During the reaction, UDP-glucose pyrophosphorylase in the sample or standard competes with a fixed amount of biotin-labeled UDP-glucose pyrophosphorylase for sites on a pre-coated Monoclonal antibody specific to UDP-glucose pyrophosphorylase. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UDP-glucose pyrophosphorylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized Product
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
每孔上样总蛋白量一致,实现样本间公平对比。 三、实战 protocol:肿瘤组织 IFN-γ 检测完整流程 以 “磁控 CAR-T 模拟细胞治疗后,肿瘤组织 IFN-γ 浓度检测” 为例,操作如下: 1.实验准备:试剂(abs9225、abs9161、abs9232、Mouse IFN-γ ELISA Kit 等)、仪器(组织处理器、高速冷冻离心机、酶标仪等)、治疗组与对照组小鼠肿瘤组织。 2. 组织裂解:肿瘤组织离体后用 PBS 冲洗吸干,精准称量 10mg 放入研磨管;加入 100μl 现配
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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