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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Polyadenylate-binding protein 1, Pabpc1
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Polyadenylate- binding protein 1, Pabpc1 ELISA KIT
Product Name:Mouse Polyadenylate- binding protein 1, Pabpc1 ELISA KIT
Packing:96T
Catalog No.:ELI-21806m
Gene Name:Mouse Pabpc1
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Pabpc1
Alternative Name:Mouse Polyadenylate-binding protein 1, Pabpc1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Polyadenylate- binding protein 1, Pabpc1 ELISA KIT allows for the in vitro quantitative determination of Mouse Pabpc1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Polyadenylate- binding protein 1, Pabpc1 ELISA KIT has been pre-coated with an Mouse Polyadenylate-binding protein 1, Pabpc1 antibody specific to Mouse Pabpc1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pabpc1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pabpc1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pabpc1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
is available in which the albumin binding site has been deleted. Agarose beads coated with these proteins will bind antibodies and serve to immunoprecipitate antigens from solution. There are differences in the affinity of these proteins for antibodies
PCR DNA-Array Profiling of DNA-Binding Transcription Factor Activities in Adult Mouse Tissues
Differential gene expression is tightly controlled by transcription factors (TFs), which bind close to target genes and interact together to activate and coregulate transcription. Bioinformatics analysis of published genome-wide gene
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
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