Phosphoglycerate kinase 1, Pgk

Mouse Phosphoglycerate kinase 1, PGK1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Phosphoglycerate kinase 1,Cell migration-inducing gene 10 protein,Primer recognition protein 2,PRP 2,PGK1,HEL-S-68p; MIG10; PGKA; PRP 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; primer recognition protein 2; phosphoglycerate kinase 1

Search name

Mouse Phosphoglycerate kinase 1 ELISA KIT ,Mouse Cell migration-inducing gene 10 protein ELISA KIT ,Mouse Primer recognition protein 2 ELISA KIT ,Mouse PRP 2 ELISA KIT ,Mouse PGK1 ELISA KIT ,Mouse HEL-S-68p ELISA KIT ,Mouse MIG10 ELISA KIT ,Mouse PGKA ELISA KIT ,Mouse PRP 2 ELISA KIT ,Mouse cell migration-inducing gene 10 protein ELISA KIT ,Mouse epididymis secretory sperm binding protein Li 68p ELISA KIT ,Mouse primer recognition protein 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Phosphoglycerate kinase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate kinase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Phosphoglycerate kinase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphoglycerate kinase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphoglycerate kinase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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