- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-16009m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Phosphoglycerate kinase 1, Pgk1
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Phosphoglycerate kinase 1, PGK1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Phosphoglycerate kinase 1,Cell migration-inducing gene 10 protein,Primer recognition protein 2,PRP 2,PGK1,HEL-S-68p; MIG10; PGKA; PRP 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; primer recognition protein 2; phosphoglycerate kinase 1
Search name
Mouse Phosphoglycerate kinase 1 ELISA KIT ,Mouse Cell migration-inducing gene 10 protein ELISA KIT ,Mouse Primer recognition protein 2 ELISA KIT ,Mouse PRP 2 ELISA KIT ,Mouse PGK1 ELISA KIT ,Mouse HEL-S-68p ELISA KIT ,Mouse MIG10 ELISA KIT ,Mouse PGKA ELISA KIT ,Mouse PRP 2 ELISA KIT ,Mouse cell migration-inducing gene 10 protein ELISA KIT ,Mouse epididymis secretory sperm binding protein Li 68p ELISA KIT ,Mouse primer recognition protein 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Phosphoglycerate kinase 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphoglycerate kinase 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Phosphoglycerate kinase 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phosphoglycerate kinase 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphoglycerate kinase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验【申请上传】Current Protocols in Immunology
and Human Interleukin 9 Unit 6.14 Measurement of Mouse and Human Interleukin 10 Unit 6.15 Measurement of Human Interleukin 11 Unit 6.16 Measurement of Human and Mouse Interleukin-12 Unit 6.17 Measurement of Human and Murine Stem Cell Factor (c-kit Ligand
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
(GAPD), mRNA 828 *NM_000291 Homo sapiens phosphoglycerate kinase 1 (PGK1), mRNA 2727 *NM_005566 Homo sapiens lactate dehydrogenase A (LDHA), mRNA 2105 *NM_002954 Homo sapiens ribosomal protein S27a (RPS27A), mRNA 4156 *NM_000981 Homo sapiens
Lentiviral Vector Mediated Transgenesis
, and can be either ubiquitous (human phosphoglycerate kinase, hPGK; ubiquitin, UbC) or tissue specific (e.g., calmodulin‐dependent protein kinase II, CamKII; liver‐specific transthyretin, mTTR). IRES refers to the internal ribosome entry site of the encephalomyocarditis virus
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