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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Protein prune homolog, Prune
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Protein prune homolog, Prune ELISA KIT
Product Name:Mouse Protein prune homolog, Prune ELISA KIT
Packing:96T
Catalog No.:ELI-15639m
Gene Name:Mouse Prune
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Prune
Alternative Name:Mouse Protein prune homolog, Prune
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Protein prune homolog, Prune ELISA KIT allows for the in vitro quantitative determination of Mouse Prune concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Protein prune homolog, Prune ELISA KIT has been pre-coated with an Mouse Protein prune homolog, Prune antibody specific to Mouse Prune .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Prune and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Prune , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Prune in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
), nuclear gene encoding mitochondrial protein, mRNA 2307 NM_001654 Homo sapiens v-raf murine sarcoma 3611 viral oncogene homolog 1 (ARAF1), mRNA 846 NM_002967 Homo sapiens scaffold attachment factor B (SAFB ), mRNA 545 NM_001183 Homo sapiens ATPase
删除突变的drs基因分析揭示,C末端区以及3个一致重复的N末端区都出现凋亡。Caspase-12、Caspase –9以及Caspase-3都继续被drs激活,Caspase--3,和Caspase-9的抑制剂都抑制drs引起的凋亡。在凋亡过程中没有看到因drs引起线粒体细胞色素C释放到细胞质去,这表明线粒体途径不是drs介导的凋亡,而且,研究人员发现,Drs蛋白能与定位在内质网的凋亡蛋白ASY/Nogo-B/RTN-x(S)结合,并且这些基因共同表达增加了凋亡的效果。这项研究结果提示,由ASY
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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