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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Origin recognition complex subunit 4, Orc4
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Origin recognition complex subunit 4, Orc4 ELISA KIT
Product Name:Mouse Origin recognition complex subunit 4, Orc4 ELISA KIT
Packing:96T
Catalog No.:ELI-14750m
Gene Name:Mouse Orc4
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Orc4
Alternative Name:Mouse Origin recognition complex subunit 4, Orc4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Origin recognition complex subunit 4, Orc4 ELISA KIT allows for the in vitro quantitative determination of Mouse Orc4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Origin recognition complex subunit 4, Orc4 ELISA KIT has been pre-coated with an Mouse Origin recognition complex subunit 4, Orc4 antibody specific to Mouse Orc4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Orc4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Orc4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Orc4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Nup153 (1) NM_005124 2593-2615 aaggcagactctaccaaatgt Human HeLa View, Order View Origin recognition complex protein 6 (Orc6) (46) AAUGGUAGCCACAUCCGGUGU HeLa View, Order View Origin
cells/mL) were incubated with LPS (2.5 μg/mL) in the presence or absence of hydroquinone for 6 hours. Supernatants were assayed for the levels of TNF-α, IL-1β, and IL-6 using a mouse TNF-α ELISA kit (Amersham, Little Chalfont, Buckinghamshire, UK).2
ArrayExpress http://www.ebi.ac.uk/arrayexpress Public collection of microarray gene expression data 17. Axeldb http://www.dkfz-heidelberg.de/abt0135/axeldb.htm Gene expression in Xenopus 18. BodyMap http://bodymap.ims.u-tokyo.ac.jp/ Human and mouse
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