Ligand of Numb protein X 2, Ln
x2 ELISA KIT/ Mouse Ligand of Numb protein X 2, Lnx2 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-14285m
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
Ligand of Numb protein X 2, Lnx2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Ligand of Numb protein X 2, Lnx2 ELISA KIT
Product Name:Mouse Ligand of Numb protein X 2, Lnx2 ELISA KIT
Packing:96T
Catalog No.:ELI-14285m
Gene Name:Mouse Lnx2
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Lnx2
Alternative Name:Mouse Ligand of Numb protein X 2, Lnx2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Ligand of Numb protein X 2, Lnx2 ELISA KIT allows for the in vitro quantitative determination of Mouse Lnx2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Ligand of Numb protein X 2, Lnx2 ELISA KIT has been pre-coated with an Mouse Ligand of Numb protein X 2, Lnx2 antibody specific to Mouse Lnx2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Lnx2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Lnx2 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Lnx2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Immunohistochemisty-Fluorescence Protocol
the cells twice by centrifugation in Wash Buffer-Saponin (400 x g for 5 minutes). Incubate cells for 30 minutes at room temperature with 15 µL of a fluorochrome-labeled secondary antibody (either FITC-labeled anti-mouse IgG1, IgG2A or IgG2B diluted
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
specificity of each fusion protein was tested by ELISA using a panel of DNA substrates (Fig. 2B,C). The predicted DNA binding site of each TFZF was decoded from the -helical sequence of the corresponding zinc finger (Table 1). As expected, the majority
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