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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
Chromosome Conformation Capture (3C) (PROT05)
volume of 1.6% and heat to 65°C for 20 minutes; Take a 2µg aliquot of chromatin, make up to 800µl with ligation buffer (final conc. 2.5ng/µl); Add Triton-X to a final conc. of 1% (40µl of 20% triton). Incubate for 1 hour at 37°C; Lower temperature to 16°
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