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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Serine
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Serine/threonine- protein kinase 31, Stk31 ELISA KIT
Product Name:Mouse Serine/threonine- protein kinase 31, Stk31 ELISA KIT
Packing:96T
Catalog No.:ELI-13829m
Gene Name:Mouse Stk31
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Stk31
Alternative Name:Mouse Serine/threonine-protein kinase 31, Stk31
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine/threonine- protein kinase 31, Stk31 ELISA KIT allows for the in vitro quantitative determination of Mouse Stk31 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Serine/threonine- protein kinase 31, Stk31 ELISA KIT has been pre-coated with an Mouse Serine/threonine-protein kinase 31, Stk31 antibody specific to Mouse Stk31 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Stk31 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Stk31 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Stk31 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
.C., Asara, J.M., Lane, W.S., Garner, C.C., and Lienhard, G.E. 2002. A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase‐activating protein (GAP) domain. J. Biol. Chem. 277:22115‐22118
kinases activate the serine/threonine kinases M n k l and M n k 2 . EMBO J. 16, 1909-1920. 13. Fujita, H., Fujita, H., Takemura, M., et al. (2003) A n ArabidopsisM A D S - b o x protein, A G L 2 4 , is specifically bound to and phosphorylated
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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