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- 详细信息
- 文献和实验
- 技术资料
- 库存:
10
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Inactive serine protease 45, Prss45
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Inactive serine protease 45, Prss45 ELISA KIT
Product Name:Mouse Inactive serine protease 45, Prss45 ELISA KIT
Packing:96T
Catalog No.:ELI-12784m
Gene Name:Mouse Prss45
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Mouse Prss45
Alternative Name:Mouse Inactive serine protease 45, Prss45
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Inactive serine protease 45, Prss45 ELISA KIT allows for the in vitro quantitative determination of Mouse Prss45 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Inactive serine protease 45, Prss45 ELISA KIT has been pre-coated with an Mouse Inactive serine protease 45, Prss45 antibody specific to Mouse Prss45 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Prss45 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Prss45 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Prss45 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验were isolated from EHS using PBS/EDTA, and also cross-linked as cells growth in 2D. 2, Wash cells twice using ice cold PBS containing protease inhibitors (1mM phenylmethylsulfonyl fluoride (PMSF), 1microgram/ml aprotinin and 1microgram/ml pepstatin
MATERIALS T-cell: CD2 IgG2b, 01171D B-cell: B220 (CD45R) IgG2a, 01121A CD3 IgG2b, 28001D Monocytic: Mac1 (CD11b) IgG2b, 01711A CD5 IgG2a, 01031
Enrichment and Clonal Culture of Hepatic Stem/Progenitor Cells During Mouse Liver Development
livers. FACS clone-sorted single CD45− Ter119− c-kit− CD13+ CD133+ cells (from fetal mid-gestational livers) or CD45− Ter119− c-kit− Sca1− CD13+ CD49f+ CD133+ cells (from adult livers) can form a colony containing both albumin-positive hepatocytes
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