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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Kinesin-1 heavy chain, Kif5b含量
- 适应物种:
小鼠
- 标记物:
Kinesin-1 heavy chain, Kif5b
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Kinesin- 1 heavy chain, Kif5b ELISA KIT
Product Name:Mouse Kinesin- 1 heavy chain, Kif5b ELISA KIT
Packing:96T
Catalog No.:ELI-08754m
Gene Name:Mouse Kif5b
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Kif5b
Alternative Name:Mouse Kinesin-1 heavy chain, Kif5b
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Kinesin- 1 heavy chain, Kif5b ELISA KIT allows for the in vitro quantitative determination of Mouse Kif5b concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Kinesin- 1 heavy chain, Kif5b ELISA KIT has been pre-coated with an Mouse Kinesin-1 heavy chain, Kif5b antibody specific to Mouse Kif5b .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Kif5b and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Kif5b , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Kif5b in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验1 fibers in immunofluorescence and with rat type 1 MHCs (myosin heavy chain)in Western blotting;the cell line was established in 1986 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse that had been immunized with purified myosin
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
me why this works!) before cloning out for variants. b) You can screen by ELISA or with mAb coupled RBC using mouse monoclonal antibodies against the Y3 derived Kappa 1a (RG11/15.5) and the specific DA Kappa 1b (G9/1.3), or the appropriate heavy chain (eg NORIG
(e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine
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