NF-kappa-B-activating protein,
Nkap ELISA KIT/ Mouse NF-kappa-B-activating protein, Nkap ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-13129m
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
NF-kappa-B-activating protein, Nkap
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse NF- kappa- B- activating protein, Nkap ELISA KIT
Product Name:Mouse NF- kappa- B- activating protein, Nkap ELISA KIT
Packing:96T
Catalog No.:ELI-13129m
Gene Name:Mouse Nkap
Detect Range:15.62-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Nkap
Alternative Name:Mouse NF-kappa-B-activating protein, Nkap
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse NF- kappa- B- activating protein, Nkap ELISA KIT allows for the in vitro quantitative determination of Mouse Nkap concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse NF- kappa- B- activating protein, Nkap ELISA KIT has been pre-coated with an Mouse NF-kappa-B-activating protein, Nkap antibody specific to Mouse Nkap .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Nkap and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Nkap , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Nkap in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
TRAF‐Mediated TNFR‐Family Signaling
Croston, G.E., Cao, Z., and Goeddel, D.V. 1995. NF‐kappa B activation by interleukin‐1 (IL‐1) requires an IL‐1 receptor‐associated protein kinase activity. J. Biol. Chem. 270:16514‐16517
Mouse Models of Bariatric Surgery
. The mouse RYGB bypasses the whole stomach and duodenum. E‐J, esophago‐jejunal anastomosis; JJ, jejuno‐jejunal anastomosis. (B ) Illustration of RYGB. View Image
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