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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白含量
- 适应物种:
小鼠
- 标记物:
Autophagy-related protein 13, Atg13
- 样本:
小鼠血清,血浆,组织匀浆
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Autophagy- related protein 13, Atg13 ELISA KIT
Product Name:Mouse Autophagy- related protein 13, Atg13 ELISA KIT
Packing:96T
Catalog No.:ELI-12014m
Gene Name:Mouse Atg13
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Atg13
Alternative Name:Mouse Autophagy-related protein 13, Atg13
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Autophagy- related protein 13, Atg13 ELISA KIT allows for the in vitro quantitative determination of Mouse Atg13 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Autophagy- related protein 13, Atg13 ELISA KIT has been pre-coated with an Mouse Autophagy-related protein 13, Atg13 antibody specific to Mouse Atg13 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Atg13 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Atg13 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Atg13 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using BLAT to Find Sequence Similarity in Closely Related Genomes
) is used to find genomic sequences that match a protein or DNA sequence submitted by the user. BLAT is typically used for searching similar sequences within the same or closely related species. It was developed to align millions of expressed sequence tags
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
, immunostaining of the chromosomes with a protein of interest, and counterstaining with Giemsa and Hoechst. RELATED INFORMATION Technical modifications incorporated into this protocol that have enabled more reproducible patterns of banding
Staining Whole Mouse Embryos for β-Galactosidase (lacZ) Activity
) activity using X-gal. RELATED INFORMATION A protocol for Staining Frozen Mouse Embryo Sections for β-Galactosidase (lacZ) Activity is also available. MATERIALS Reagents Detergent rinse Embryo staining solution
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