- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-11428m
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Aurora kinase C, Aurkc含量
- 适应物种：
小鼠
- 标记物：
Aurora kinase C, Aurkc
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Aurora kinase C, Aurkc ELISA KIT
Product Name:Mouse Aurora kinase C, Aurkc ELISA KIT
Packing:96T
Catalog No.:ELI-11428m
Gene Name:Mouse Aurkc
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Aurkc
Alternative Name:Mouse Aurora kinase C, Aurkc
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Aurora kinase C, Aurkc ELISA KIT allows for the in vitro quantitative determination of Mouse Aurkc concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Aurora kinase C, Aurkc ELISA KIT has been pre-coated with an Mouse Aurora kinase C, Aurkc antibody specific to Mouse Aurkc .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Aurkc and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Aurkc , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Aurkc in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Studying the Roles of Aurora-C Kinase During Meiosis in Mouse Oocytes
We previously isolated Aurora-C (Aurkc/Aie1 ) in a screen for kinases expressed in mouse sperm and eggs. Aurora-C kinase was reported to be a chromosomal passenger protein that plays critical roles in chromosome alignment
licanming 请问有无人用过assaydesigns公司的PKC Kinase Activity Assay Kit（cat NO:EKS-420A）?可否说一下应用的经验？注意事项等？还有，该试剂盒的说明书有几处地方不是很懂，请大家帮忙看一下： 1、To avoid cross contamination, change disposable pipette tips between the addition of each standard
运用Cell Based Elisa检测信号通路蛋白和磷酸化蛋白
using Phospho-p38 and Total-p38 antibodies (C). Cell-Based Elisa将小鼠巨噬细胞4/4细胞种在96孔板内，每个孔种5 x 104 cells/cm2细胞。细胞用不同浓度的anisomycin刺激，我们来监测P38 MAPK和JNK两种蛋白的变化。按照试剂盒的实验步骤，分别固定，打孔，猝灭，封闭，然后一抗孵育，二抗孵育，最后显色，用常规的酶标仪读取数值。最后数据再和试剂盒提供的结晶紫核染料结果做纠正，最后得出以上数据。Quantitative
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