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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Calcium-binding protein 39-like, Cab39l含量
- 适应物种:
小鼠
- 标记物:
Calcium-binding protein 39-like, Cab39l
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Calcium- binding protein 39- like, Cab39l ELISA KIT
Product Name:Mouse Calcium- binding protein 39- like, Cab39l ELISA KIT
Packing:96T
Catalog No.:ELI-10895m
Gene Name:Mouse Cab39l
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cab39l
Alternative Name:Mouse Calcium-binding protein 39-like, Cab39l
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Calcium- binding protein 39- like, Cab39l ELISA KIT allows for the in vitro quantitative determination of Mouse Cab39l concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Calcium- binding protein 39- like, Cab39l ELISA KIT has been pre-coated with an Mouse Calcium-binding protein 39-like, Cab39l antibody specific to Mouse Cab39l .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cab39l and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cab39l , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cab39l in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
Practical Aspects of Radioligand Binding
) is 0.232 nM, and the B max (the x intercept) is 61 fmol/mg. The data in Figure were normalized to mg protein, whereas the data in Figure are expressed in fmol/tube specific binding. View Image
Using Biacore to Measure the Binding Kinetics of an Antibody‐Antigen Interaction
?protein interaction is described using an antibody and its antigen as an example. The affinity of the antibody for its antigen is determined by measuring the binding kinetics of the interaction. The protocols are divided into three major steps
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