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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白G2含量
- 适应物种:
小鼠
- 标记物:
G2
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse G2/mitotic- specific cyclin- B3, Ccnb3 ELISA KIT
Product Name:Mouse G2/mitotic- specific cyclin- B3, Ccnb3 ELISA KIT
Packing:96T
Catalog No.:ELI-10675m
Gene Name:Mouse Ccnb3
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Ccnb3
Alternative Name:Mouse G2/mitotic-specific cyclin-B3, Ccnb3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse G2/mitotic- specific cyclin- B3, Ccnb3 ELISA KIT allows for the in vitro quantitative determination of Mouse Ccnb3 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse G2/mitotic- specific cyclin- B3, Ccnb3 ELISA KIT has been pre-coated with an Mouse G2/mitotic-specific cyclin-B3, Ccnb3 antibody specific to Mouse Ccnb3 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ccnb3 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ccnb3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ccnb3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验,or write to the author. Identification of Tissue-Specific MicroRNAs from Mouse http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRT-45X0D1F-N&_user=440026&_coverDate=04%2F30%2F2002&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c
【转贴】immunofluorescence staining
well for many other cell lines. However, some cells may require adjustments in length of incubations, and concentration of formaldehyde or Triton X-100.Part 1: Initial Fixation, with or without extractionAspirate or pipette off media. Wash X 2 with ice-cold PBS (containing
A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
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