G2/mitotic-specific cyclin-B1,
Ccnb1 ELISA KIT/ Mouse G2/mitotic-specific cyclin-B1, Ccnb1 ELISA试剂盒- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-34238m
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白含量
- 适应物种：
小鼠
- 标记物：
G2
- 样本：
小鼠血清，血浆，组织匀浆
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse G2/mitotic- specific cyclin- B1, Ccnb1 ELISA KIT
Product Name:Mouse G2/mitotic- specific cyclin- B1, Ccnb1 ELISA KIT
Packing:96T
Catalog No.:ELI-34238m
Gene Name:Mouse Ccnb1
Detect Range:15.6-1000pg/ml
Sensitivity:9.375pg/ml
Target Protein Name:Mouse Ccnb1
Alternative Name:Mouse G2/mitotic-specific cyclin-B1, Ccnb1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse G2/mitotic- specific cyclin- B1, Ccnb1 ELISA KIT allows for the in vitro quantitative determination of Mouse Ccnb1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse G2/mitotic- specific cyclin- B1, Ccnb1 ELISA KIT has been pre-coated with an Mouse G2/mitotic-specific cyclin-B1, Ccnb1 antibody specific to Mouse Ccnb1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ccnb1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ccnb1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ccnb1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验,or write to the author. Identification of Tissue-Specific MicroRNAs from Mouse http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6VRT-45X0D1F-N&_user=440026&_coverDate=04%2F30%2F2002&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c
with 2 steps: in vivo formaldehyde cross-linking of whole cells and protein-protein and protein-DNA interactions, followed by immunoprecipitation of protein-DNA complexes with specific antibodies from sonicated extracts. Breeden Lab Chromatin-IP (ChrIP
【转贴】immunofluorescence staining
well for many other cell lines. However, some cells may require adjustments in length of incubations, and concentration of formaldehyde or Triton X-100.Part 1: Initial Fixation, with or without extractionAspirate or pipette off media. Wash X 2 with ice-cold PBS (containing
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