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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Leukocyte antigen CD37, Cd37含量
- 适应物种:
小鼠
- 标记物:
Leukocyte antigen CD37, Cd37
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Leukocyte antigen CD37, Cd37 ELISA KIT
Product Name:Mouse Leukocyte antigen CD37, Cd37 ELISA KIT
Packing:96T
Catalog No.:ELI-10383m
Gene Name:Mouse Cd37
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Cd37
Alternative Name:Mouse Leukocyte antigen CD37, Cd37
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Leukocyte antigen CD37, Cd37 ELISA KIT allows for the in vitro quantitative determination of Mouse Cd37 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Leukocyte antigen CD37, Cd37 ELISA KIT has been pre-coated with an Mouse Leukocyte antigen CD37, Cd37 antibody specific to Mouse Cd37 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Cd37 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Cd37 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Cd37 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验80年代初以来,由于单克隆抗体,分子克隆、基因转染细胞系等技术在白细胞分化抗原研究中得到广泛深入的应用,有关白细胞分化抗原的研究和应用进展相当迅速。在世界卫生组织(WHO)和国际免疫学会联合会(IUIS)的组织下,自1982年至1993年已先后举行了五次有关人类白细胞分化抗原的国际协作组会议(International workshop on human leukocyte differentiation
,2F12;CRIS-3 Leu LFA-1(gp180/95)的α链 与ICAM-1(CD54)和ICAM-2(CD102)结合 CD11b Mol,OKM1; (Mac-1,CR3,integrin αm G,M,NK gp165/95的α链 C3bi和FB受体,与ICAM-1结合,粘附,调理吞噬 CD11c LeuM5;(CR4,integrin αx) M,G,NK,Tsub gp150/95的α链 C3bi受体,调理吞噬
Immunohistochemistry: Fluorescence Protocol 2.0
by centrifugation (400 x g for 5 minutes). Incubate the cells for 30 minutes at room temperature with 50 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted 1:700 or biotin-goat anti-mouse IgG1 or IgG2A or IgG2B diluted
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