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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Elongation factor 1-alpha 1, Eef1a1含量
- 适应物种:
小鼠
- 标记物:
Elongation factor 1-alpha 1, Eef1a1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Elongation factor 1- alpha 1, Eef1a1 ELISA KIT
Product Name:Mouse Elongation factor 1- alpha 1, Eef1a1 ELISA KIT
Packing:96T
Catalog No.:ELI-09608m
Gene Name:Mouse Eef1a1
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Eef1a1
Alternative Name:Mouse Elongation factor 1-alpha 1, Eef1a1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Elongation factor 1- alpha 1, Eef1a1 ELISA KIT allows for the in vitro quantitative determination of Mouse Eef1a1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Elongation factor 1- alpha 1, Eef1a1 ELISA KIT has been pre-coated with an Mouse Elongation factor 1-alpha 1, Eef1a1 antibody specific to Mouse Eef1a1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Eef1a1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Eef1a1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Eef1a1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
Homo sapiens matrix metalloproteinase-like 1 (MMPL1), mRNA 695 NM_001950 Homo sapiens E2F transcription factor 4, p107/p130-binding (E2F4), mRNA 956 NM_003815 Homo sapiens a disintegrin and metalloproteinase domain 15 (metargidin) (ADAM15), mRNA
Single-molecule analysis of DNA replication by molecular combing
) and incubate for 48 hours at 50°C. Wash 5 x 10 min in 10 ml of 10 mM Tris, 50 mM EDTA and store plugs at 4°C. DNA combing DNA combing is performed on silanized coverslips essentially as described previously by the Bensimon lab (Michalet et al
was unresponsive to the transcription factors (Fig. 4D). The sequence 5'-GGAA-3' (ETS-binding site-2, EBS2) is conserved between mouse and the human promoters, and in the mouse it interacts with the ETS-1 protein, a transcription factor of the ETS family expressed
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