Dynein heavy chain 17, axonema

Mouse Dynein heavy chain 17 axonemal, Dnah17 ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Dynein heavy chain 17, axonemal,Dynein light chain 2, axonemal,Ciliary dynein heavy chain-like protein 1,Ciliary dynein heavy chain 17,Axonemal beta dynein heavy chain 17,Axonemal dynein heavy chain-like protein 1, DYH17,DNAH17, DNAHc17, DNAHL1; DNEL2; axonemal beta dynein heavy chain 17; axonemal dynein heavy chain; axonemal dynein heavy chain-like protein 1; ciliary dynein heavy chain 17; ciliary dynein heavy chain-like protein 1; dynein heavy chain 17, axonemal; dynein light chain 2, axonemal; dynein, axonemal, heavy chain like 1; dynein, axonemal, heavy like 1; dynein, axonemal, heavy polypeptide 17; dynein, axonemal, heavy chain 17,Kiaa3028

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse dynein heavy chain 17, axonemal,DYH17 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DYH17, During the reaction, DYH17 in the sample or standard competes with a fixed amount of biotin-labeled DYH17 for sites on a pre-coated Monoclonal antibody specific to DYH17. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DYH17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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