- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-08804m
- 2025年07月03日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Histone H3.3C, H3f3c含量
- 适应物种：
小鼠
- 标记物：
Histone H3.3C, H3f3c
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Histone H3.3C, H3f3c ELISA KIT
Product Name:Mouse Histone H3.3C, H3f3c ELISA KIT
Packing:96T
Catalog No.:ELI-08804m
Gene Name:Mouse H3f3c
Detect Range:78-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse H3f3c
Alternative Name:Mouse Histone H3.3C, H3f3c
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Histone H3.3C, H3f3c ELISA KIT allows for the in vitro quantitative determination of Mouse H3f3c concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Histone H3.3C, H3f3c ELISA KIT has been pre-coated with an Mouse Histone H3.3C, H3f3c antibody specific to Mouse H3f3c .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse H3f3c and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse H3f3c , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse H3f3c in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验3X Flag Peptide) to each pelleted bead/complex, incubate 30-60 minutes at 4°C with gentle shaking;Centrifuge the resin for 30 seconds at 10,000 RPM, transfer the supernatants (which should now contain the ChIP/Flag eluted complexes) to fresh tubes
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
of 0.3% SDS). Mix by gentle pipetting. Shake gently for 1 h at 37°C. 15. Add 50 µL of 20% Triton X-100 (to a final concentration of 1.8%). Shake gently for 1 h at 37°C. 16. Add 1200 units of restriction enzyme (i.e., 12 µL
Identifying Differential Histone Modification Sites from ChIPseq Data
a hidden Markov model (HMM) to infer the states of histone modification changes at each genomic location. We evaluated the performance of ChIPDiff by comparing the H3K27me3 modification sites between mouse embryonic stem cell (ESC) and neural progenitor
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