- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-07532m
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Interferon alpha-7, Ifna7含量
- 适应物种：
小鼠
- 标记物：
Interferon alpha-7, Ifna7
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Interferon alpha- 7, Ifna7 ELISA KIT
Product Name:Mouse Interferon alpha- 7, Ifna7 ELISA KIT
Packing:96T
Catalog No.:ELI-07532m
Gene Name:Mouse Ifna7
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ifna7
Alternative Name:Mouse Interferon alpha-7, Ifna7
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Interferon alpha- 7, Ifna7 ELISA KIT allows for the in vitro quantitative determination of Mouse Ifna7 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Interferon alpha- 7, Ifna7 ELISA KIT has been pre-coated with an Mouse Interferon alpha-7, Ifna7 antibody specific to Mouse Ifna7 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ifna7 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ifna7 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ifna7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验motifs located prevalently in the 3′ untranslated regions of target messenger RNAs (mRNA). Interferon-alpha-2a (IFNα) induces a large set of protein coding genes mediating antiproliferative and antiviral responses. Here we use a global microarray-based
Measurement of Mouse Cytomegalovirus-Induced Interferon- with Immortalized Luciferase Reporter Cells
genes. We describe here the generation and immortalization of cells derived from transgenic reporter mice for development of a high-throughput assay system for virus- or bacteria-induced cytokine induction. As an example we describe mouse cytomegalovirus
CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
部分。它们的错误折叠，如天然结构的丢失，是任何分析的一个重大问题，因为它会引起抗体结合部位、抗体的抗原结合区或抗原的表位的改变，这两者都是待测物结合所必需的。因此，捕获分子无法结合待测物，甚至可能由于暴露天然未暴露的氨基酸序列和结构而发生非特异性结合，导致诊断试验显示错误的结果。由于表面效应引起的空间结构和构象的改变，抗体在包被过程中会发生错误折叠。在ELISA微孔板中，只有3-10%的包被抗体具有良好的定向性和与待测物结合的功能活性。抗原包被也有类似的情况。在大多数情况下，很少量的活性成分就足以完成一次检测
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