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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Transcriptional repressor CTCF, Ctcf含量
- 适应物种:
小鼠
- 标记物:
Transcriptional repressor CTCF, Ctcf
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Transcriptional repressor CTCF, Ctcf ELISA KIT
Product Name:Mouse Transcriptional repressor CTCF, Ctcf ELISA KIT
Packing:96T
Catalog No.:ELI-07425m
Gene Name:Mouse Ctcf
Detect Range:78.125-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Ctcf
Alternative Name:Mouse Transcriptional repressor CTCF, Ctcf
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Transcriptional repressor CTCF, Ctcf ELISA KIT allows for the in vitro quantitative determination of Mouse Ctcf concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Transcriptional repressor CTCF, Ctcf ELISA KIT has been pre-coated with an Mouse Transcriptional repressor CTCF, Ctcf antibody specific to Mouse Ctcf .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Ctcf and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Ctcf , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Ctcf in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Developing Genetically Engineered Mouse Models to Study Tumor Suppression
Costantini, F. and Lacy, E. 1981. Introduction of a rabbit beta‐globin gene into the mouse germ line. Nature 294:92‐94. Cui, X., Ji, D., Fisher, D.A., Wu, Y
Mapping Networks of Protein‐Mediated Physical Interactions Between Chromatin Elements
Ling, J.Q., Li, T., Hu, J.F., Vu, T.H., Chen, H.L., Qiu, X.W., Cherry, A.M., and Hoffman, A.R. 2006. CTCF mediates interchromosomal colocalization between Igf2/H19 and Wsb1/Nf1. Science 312:269‐272.
, such as RNA interference, ribozymes, or antisense RNA, that provide solely knock-down phenotypes2, 3 , transcription factor�based tools can generate both loss-of-function phenotypes (when the transcription factor is linked to a repressor domain) and gain
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