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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白X-box-binding protein 1, Xbp1含量
- 适应物种:
小鼠
- 标记物:
X-box-binding protein 1, Xbp1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse X- box- binding protein 1, Xbp1 ELISA KIT
Product Name:Mouse X- box- binding protein 1, Xbp1 ELISA KIT
Packing:96T
Catalog No.:ELI-07690m
Gene Name:Mouse Xbp1
Detect Range:0.156-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Xbp1
Alternative Name:Mouse X-box-binding protein 1, Xbp1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse X- box- binding protein 1, Xbp1 ELISA KIT allows for the in vitro quantitative determination of Mouse Xbp1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse X- box- binding protein 1, Xbp1 ELISA KIT has been pre-coated with an Mouse X-box-binding protein 1, Xbp1 antibody specific to Mouse Xbp1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Xbp1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Xbp1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Xbp1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Isolate It All:siRNA • miRNA • Total RNA • Native Protein
Figure 5. Isolation of Total RNA and Protein from Different Tissues. Total RNA and protein were isolated from 4 different mouse tissues (~50 mg) with the mir Vana™ miRNA Isolation Kit (RNA only) or the mir Vana™ PARIS™ Kit. (A) Small RNA
ChIP‐chip for Genome‐Wide Analysis of Protein Binding in Mammalian Cells
Analysis of Protein‐DNA Binding Sites Basic Protocol 2: Analysis of PCR Array Data Support Protocol 1: Preparation of Annealed Linkers Reagents and Solutions Commentary
DamIP: Using Mutant DNA Adenine Methyltransferase to Study DNA‐Protein Interactions In Vivo
for studying DNA?protein interaction in vivo. A mutant form of DNA adenine methyltransferase (DamK9A) from E. coli is fused to the protein of interest and expressed. The fusion protein will bind to target binding sites and introduce N 6 ?adenine methylation
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