- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-07056m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Extracellular superoxide dismutase [Cu-Zn], Sod3 ELISA KI含量
- 适应物种：
小鼠
- 标记物：
Extracellular superoxide dismutase [Cu-Zn], Sod3 ELISA KI
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Extracellular superoxide dismutase [Cu- Zn], SOD3 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Class 1.15.1.1, Superoxide dismutase B, SOD3,EC-SOD, extracellular superoxide dismutase [Cu-Zn],superoxide dismutase 3 extracellular
Search name
Mouse Class 1.15.1.1 ELISA KIT ,Mouse Superoxide dismutase B ELISA KIT ,Mouse SOD3 ELISA KIT ,Mouse EC-SOD ELISA KIT ,Mouse extracellular superoxide dismutase [Cu-Zn] ELISA KIT ,Mouse superoxide dismutase 3 extracellular ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse extracellular superoxide dismutase [cu-zn],EC-SOD concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EC-SOD, During the reaction, EC-SOD in the sample or standard competes with a fixed amount of biotin-labeled EC-SOD for sites on a pre-coated Monoclonal antibody specific to EC-SOD. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EC-SOD in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验请教yong主任和各位高手,几个PCR引物设计的深层次的问题
目的：RT-PCR克隆全长目的基因做表达基因11: M25157. Rat Cu, Zn supero...[gi:207011] LinksLOCUS RATSODCZL 601 bp mRNA linear ROD 27-APR-1993DEFINITION Rat Cu, Zn superoxide dismutase mRNA, complete cds.ACCESSION M25157VERSION M25157.1 GI:207011KEYWORDS superoxide
（Assay Designs Inc.）[2].（8） ； ；SOD活性的测定采用黄嘌呤氧化酶法； 邻苯三氯自氧化法（SOD试剂盒，四川省医学试剂研究开发中心，Cu-Zn SOD南京建成生物工程研究所生产试剂盒）。Cu，Zn-Superoxide dismutase and Mn-superoxide dismutase activities were measured by using a water-soluble formazan dye kit.SOD activity was measured
Establishing In Vitro Models to Study Endogenous Neurotoxicants
Parkinson’s Lewy body dementia Lewy bodies Substantia nigra Cortical pyramidal neurons 6 ,11 Cu/Zn
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