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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Superoxide dismutase [Cu-Zn], Sod1含量
- 适应物种:
小鼠
- 标记物:
Superoxide dismutase [Cu-Zn], Sod1
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Superoxide dismutase [Cu-Zn], Sod1 ELISA试剂盒
Product Name: Mouse Superoxide dismutase [Cu-Zn], Sod1 ELISA试剂盒
Product Name: Mouse Superoxide dismutase [Cu-Zn], Sod1 ELISA试剂盒
Gene Name: Mouse Superoxide dismutase [Cu-Zn], Sod1
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Superoxide dismutase [Cu-Zn], Sod1
Alternative Name: Mouse Superoxide dismutase [Cu-Zn], Sod1
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Superoxide dismutase [Cu-Zn], Sod1 ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Superoxide dismutase [Cu-Zn], Sod1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Superoxide dismutase [Cu-Zn], Sod1 ELISA试剂盒 has been pre-coated with an Mouse Superoxide dismutase [Cu-Zn], Sod1 antibody specific to Mouse Superoxide dismutase [Cu-Zn], Sod1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Superoxide dismutase [Cu-Zn], Sod1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Superoxide dismutase [Cu-Zn], Sod1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Superoxide dismutase [Cu-Zn], Sod1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Overexpression of Chloroplastic Cu/Zn Superoxide Dismutase in Plants
stress. These protective mechanisms include both enzymatic and nonenzymatic systems. Superoxide dismutases (SODS) catalyze the vital first step in the enzymatic oxidative stress protective system.
Assay of superoxide dismutase activity
-293. The authors are grateful to Dr. Chia-Yin Tsai for helpful discussion and critical review of this manuscript. Chen and Pan ― Assay of superoxide dismutase activity Literature Cited Beauchamp, C. and I. Fridovich
Relation Between Mitochondrial Membrane Potential and ROS Formation
dismutase (MnSOD, SOD2) from the mitochondrial matrix as well as superoxide dismutase (Cu/ZnSOD, SOD1) present in small amounts in the mitochondrial intramembrane space, convert superoxide anion to hydrogen peroxide, which can be then converted by catalase
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