- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-06853m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Telomerase reverse transcriptase, Tert含量
- 适应物种：
小鼠
- 标记物：
Telomerase reverse transcriptase, Tert
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Telomerase reverse transcriptase, TERT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TERT,CMM9; DKCA2; DKCB4; EST2; PFBMFT1; TCS1; TP2; TRT; hEST2; hTRT; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase,Telomerase reverse transcriptase,Telomerase-associated protein 2,TP2,HEST2,Telomerase catalytic subunit,
Search name
Mouse TERT ELISA KIT ,Mouse CMM9 ELISA KIT ,Mouse DKCA2 ELISA KIT ,Mouse DKCB4 ELISA KIT ,Mouse EST2 ELISA KIT ,Mouse PFBMFT1 ELISA KIT ,Mouse TCS1 ELISA KIT ,Mouse TP2 ELISA KIT ,Mouse TRT ELISA KIT ,Mouse hEST2 ELISA KIT ,Mouse hTRT ELISA KIT ,Mouse telomerase catalytic subunit ELISA KIT ,Mouse telomerase-associated protein 2 ELISA KIT ,Mouse telomerase reverse transcriptase ELISA KIT ,Mouse Telomerase reverse transcriptase ELISA KIT ,Mouse Telomerase-associated protein 2 ELISA KIT ,Mouse TP2 ELISA KIT ,Mouse HEST2 ELISA KIT ,Mouse Telomerase catalytic subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Telomerase reverse transcriptase concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase reverse transcriptase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Telomerase reverse transcriptase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Telomerase reverse transcriptase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Telomerase reverse transcriptase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
depletion of unfractionated bone marrow followed by fluorescence-activated cell sorting (FACS). The HSC in lineage-negative cell populations from mouse bone marrow are then sorted for HSC using MAbs specific for surface markers such as Sca-1 or c-kit (Fig
Methods of Telomerase Inhibition
as a possible anticancer approach. Many of the methods applied to telomerase inhibition focus on either of the two major components of the ribonucleoprotein holoenzyme, that is, the telomerase reverse transcriptase (TERT) catalytic subunit or the telomerase RNA
Assessing the Involvement of Telomerase in Stem Cell Biology
) and a protein called TERT that possesses reverse transcriptase activity. Several telomere-associated proteins have been identified including TRF1 (telomere repeat-binding factor 1) that may inhibit telomerase activity and promote telomere shortening, and TRF
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