Pyrin, Mefv ELISA KIT/ Mouse P

Mouse Pyrin, MEFV ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MEFV,Mediterranean fever,FMF; MEF; TRIM20;Pyrin,Marenostrin,;

Search name

Mouse MEFV ELISA KIT ,Mouse Mediterranean fever ELISA KIT ,Mouse FMF ELISA KIT ,Mouse MEF ELISA KIT ,Mouse TRIM20 ELISA KIT, Mouse Pyrin ELISA KIT ,Mouse Marenostrin ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Pyrin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyrin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyrin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyrin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pyrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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