- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-06745m
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Pyrin, Mefv含量
- 适应物种：
小鼠
- 标记物：
Pyrin, Mefv
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Pyrin, MEFV ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MEFV,Mediterranean fever,FMF; MEF; TRIM20;Pyrin,Marenostrin,;
Search name
Mouse MEFV ELISA KIT ,Mouse Mediterranean fever ELISA KIT ,Mouse FMF ELISA KIT ,Mouse MEF ELISA KIT ,Mouse TRIM20 ELISA KIT, Mouse Pyrin ELISA KIT ,Mouse Marenostrin ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Pyrin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyrin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pyrin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Pyrin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pyrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
基因敲除模型：NLRP3−/−、GSDMD−/−、caspase-1/11−/−小鼠、RACK1ΔMΦ。 4、检测内容：LDH（乳酸脱氢酶）释放实验检测细胞毒性、ELISA 检测细胞因子分泌、WB 检测、免疫沉淀（IP）、pull down、质谱、EST12 晶体结构、共聚焦显微镜、小鼠结核感染模型等。 研究结果： 1、EST12（Rv1579c）诱导 GSDMD 介导的巨噬细胞焦亡 表达纯化 40 种 RD 区编码蛋白，通过 LDH 释放实验，发现 EST12（Rv1579c）具有明显的巨噬
Performing Bronchoalveolar Lavage in the Mouse
and inflammatory cell populations; it is indispensable for studying cell influx in disease models of the airways such as asthma and COPD. Cell counts can be combined with methods such as ELISA, immunoblot, immunohistochemistry, quantitative polymerase chain
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