- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-05938m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白NF-kappa-B inhibitor alpha, Nfkbia含量
- 适应物种：
小鼠
- 标记物：
NF-kappa-B inhibitor alpha, Nfkbia
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse NF- kappa- B inhibitor alpha, Nfkbia ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NF-kappa-B inhibitor alpha,I-kappa-B-alpha(IkB-alpha,IkappaBalpha),RL/IF-1,Nfkbia,IkappaBalpha,NF-kappa-B inhibitor alpha; IKBA; MAD-3; NFKBI; I-kappa-B-alpha; ikB-alpha; major histocompatibility complex enhancer-binding protein MAD3; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
Search name
Mouse NF-kappa-B inhibitor alpha ELISA KIT ,Mouse I-kappa-B-alpha ELISA KIT ,Mouse IkB-alpha ELISA KIT ,Mouse IkappaBalpha ELISA KIT ,Mouse RL/IF-1 ELISA KIT ,Mouse Nfkbia ELISA KIT ,Mouse IkappaBalpha ELISA KIT ,Mouse NF-kappa-B inhibitor alpha ELISA KIT ,Mouse IKBA ELISA KIT ,Mouse MAD-3 ELISA KIT ,Mouse NFKBI ELISA KIT ,Mouse I-kappa-B-alpha ELISA KIT ,Mouse ikB-alpha ELISA KIT ,Mouse major histocompatibility complex enhancer-binding protein MAD3 ELISA KIT ,Mouse nuclear factor of kappa light chain gene enhancer in B-cells ELISA KIT ,Mouse nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse NF-kappa-B inhibitor alpha concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NF-kappa-B inhibitor alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-kappa-B inhibitor alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-kappa-B inhibitor alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NF-kappa-B inhibitor alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验accumulation by LPS, as TLR4 can use either MyD88 or TRIF. The net result of these interactions was activation of the transcription factors NF-{kappa}B and AP-1, and synthesis of TNF-{alpha} mRNA. TNF-{alpha} acts through an apparent autocrine pathway
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
Performing Bronchoalveolar Lavage in the Mouse
Delayre‐Orthez, C., Becker, J., de Blay, F., Frossard, N., and Pons, F. 2005b. Exposure to endotoxins during sensitization prevents further endotoxin‐induced exacerbation of airway inflammation in a mouse model of allergic asthma. Int. Arch
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