- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04891m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase 含量
- 适应物种：
小鼠
- 标记物：
Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Phosphatidylinositol- 3,4,5- trisphosphate 3- phosphatase and dual- specificity protein phosphatase PTEN, PTEN ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PTEN,phosphatidylinositol; 10q23del; BZS; CWS1; DEC; GLM2; MHAM; MMAC1; PTEN1; TEP1; MMAC1 phosphatase and tensin homolog deleted on chromosome 10; mutated in multiple advanced cancers 1; phosphatase and tensin-like protein; phosphatase and tensin homolog,3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
Search name
Mouse PTEN ELISA KIT ,Mouse phosphatidylinositol ELISA KIT ,Mouse 10q23del ELISA KIT ,Mouse BZS ELISA KIT ,Mouse CWS1 ELISA KIT ,Mouse DEC ELISA KIT ,Mouse GLM2 ELISA KIT ,Mouse MHAM ELISA KIT ,Mouse MMAC1 ELISA KIT ,Mouse PTEN1 ELISA KIT ,Mouse TEP1 ELISA KIT ,Mouse MMAC1 phosphatase and tensin homolog deleted on chromosome 10 ELISA KIT ,Mouse mutated in multiple advanced cancers 1 ELISA KIT ,Mouse phosphatase and tensin-like protein ELISA KIT ,Mouse phosphatase and tensin homolog ELISA KIT,Mouse 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA KIT, Mouse phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN, PTEN concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTEN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PTEN and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTEN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTEN in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
phosphatase-conjugated antibodies. However, by carefully controlling the reaction time in X-gal so that only a small amount of indigo is produced (Vaysse and Goldman, 1990), by having ß-gal localized to the nucleus when the cellular antigen is non-nuclear
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
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