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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA或竞争法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Dopamine beta-hydroxylase, Dbh含量
- 适应物种:
小鼠
- 标记物:
Dopamine beta-hydroxylase, Dbh
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Dopamine beta-hydroxylase, Dbh ELISA试剂盒
Product Name: Mouse Dopamine beta-hydroxylase, Dbh ELISA试剂盒
Product Name: Mouse Dopamine beta-hydroxylase, Dbh ELISA试剂盒
Gene Name: Mouse Dopamine beta-hydroxylase, Dbh
Detect Range:78-5000pg/ml
Sensitivity:39pg/ml
Target Protein Name: Mouse Dopamine beta-hydroxylase, Dbh
Alternative Name: Mouse Dopamine beta-hydroxylase, Dbh
ELISA type:Sandwich ELISA Kit
ELISA Test Principle:
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Product Description: Mouse Dopamine beta-hydroxylase, Dbh ELISA试剂盒 allows for the in vitro quantitative determination of Mouse Dopamine beta-hydroxylase, Dbh concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
The microtiter plate provided in Mouse Dopamine beta-hydroxylase, Dbh ELISA试剂盒 has been pre-coated with an Mouse Dopamine beta-hydroxylase, Dbh antibody specific to Mouse Dopamine beta-hydroxylase, Dbh .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dopamine beta-hydroxylase, Dbh and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dopamine beta-hydroxylase, Dbh , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dopamine beta-hydroxylase, Dbh in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Dopamine β-hydroxylase [DBH; dopamine β-monooxygenase; EC l.14.17.1 3, 4-dihydroxyphenylethylamine; ascorbate: oxygen oxidoreductase (hydroxylation)] is a copper-containing monooxygenase that catalyzes hydroxylation of a phenylethylamine (mainly
Phenylalanine Hydroxylase, Tyrosine Hydroxylase, and Tryptophan Hydroxylase
There are four enzymes that utilize reduced pteridine as an electron donor and incorporate one atom of oxygen into their substrates. They are phenylalanine 4-monooxygenase (phenylalanine hydroxylase, PAH, EC 1.14.16.l), tyrosine 3-monooxygenase
Quantification of Tyrosine Hydroxylase mRNA
The main biochemical characteristic of Parkinson’s disease (PD) is reduction of the neurotransmitter dopamine and the dopamine-synthesizing enzyme system, including tyrosine hydroxylase (TH, tyrosine 3-monooxygenase, EC 1.14.16.2
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