- ￥2980
- LSM Bio
- 78-5000pg/ml
- 39pg/ml
- 进口
- ELI-04662m
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
10
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法ELISA或竞争法ELISA
- 应用：
检测小鼠血清，血浆，组织匀浆内的目标蛋白Dopamine beta-hydroxylase, Dbh含量
- 适应物种：
小鼠
- 标记物：
Dopamine beta-hydroxylase, Dbh
- 样本：
小鼠血清，血浆，组织匀浆及其他生物样本
- 灵敏度：
39pg/ml
- 规格：
96Tests
Mouse Dopamine beta- hydroxylase, DBH ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DBH,dopamine beta hydroxylase,DBM; dopamine beta-hydroxylase; dopamine beta-hydroxylase,dopamine beta-monooxygenase,
Search name
Mouse DBH ELISA KIT ,Mouse dopamine beta hydroxylase ELISA KIT ,Mouse DBM ELISA KIT ,Mouse dopamine beta-hydroxylase ELISA KIT ,Mouse dopamine beta-hydroxylase ELISA KIT ,Mouse dopamine beta-monooxygenase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse dopamine beta-hydroxylase,Dopamine beta-hydroxylase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dopamine beta-hydroxylase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dopamine beta-hydroxylase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dopamine beta-hydroxylase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dopamine beta-hydroxylase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Dopamine β-hydroxylase [DBH; dopamine β-monooxygenase; EC l.14.17.1 3, 4-dihydroxyphenylethylamine; ascorbate: oxygen oxidoreductase (hydroxylation)] is a copper-containing monooxygenase that catalyzes hydroxylation of a phenylethylamine (mainly
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
FA Extraction of [insoluble] Amyloid beta from Mouse Brain
ice. 7. Incubate at 37°C for 5 min prior to loading onto ELISA plates to clarify solution and solubilize precipitate. 注意事项 Neutralized material is usually diluted at least 1:2 with buffer EC prior to loading onto ELISA
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