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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法ELISA
- 应用:
检测小鼠血清,血浆,组织匀浆内的目标蛋白Aspartyl含量
- 适应物种:
小鼠
- 标记物:
Aspartyl
- 样本:
小鼠血清,血浆,组织匀浆及其他生物样本
- 灵敏度:
39pg/ml
- 规格:
96Tests
Mouse Aspartyl/asparaginyl beta- hydroxylase, Asph ELISA KIT
Product Name:Mouse Aspartyl/asparaginyl beta- hydroxylase, Asph ELISA KIT
Packing:96T
Catalog No.:ELI-11277m
Gene Name:Mouse Asph
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Asph
Alternative Name:Mouse Aspartyl/asparaginyl beta-hydroxylase, Asph
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Aspartyl/asparaginyl beta- hydroxylase, Asph ELISA KIT allows for the in vitro quantitative determination of Mouse Asph concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Aspartyl/asparaginyl beta- hydroxylase, Asph ELISA KIT has been pre-coated with an Mouse Aspartyl/asparaginyl beta-hydroxylase, Asph antibody specific to Mouse Asph .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Asph and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Asph , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Asph in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
endothelial growth factor B (VEGFB ), mRNA 649 NM_003576 Homo sapiens serine/threonine kinase 24 (STE20 homolog, yeast) (STK24), mRNA 769 NM_000918 Homo sapiens procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide
a subset of genomic DNA sequences of type 5'-(NNN )3 -3', whereas the 6ZF library would recognize a subset of genomic sequences of type 5'-(NNN )6 -3'. Given the zinc-finger domains used, both libraries were more likely to recognize (RNN)x -type sequences
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